Mass Spectrometry Facility

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Please fill out the sample form before submitting a sample.

Please contact us to schedule an analysis time.

 

Sample Preparation:

 

MALDI-TOF:  see the MALDI sample preparation guide.  PROWL also lists several MALDI preparation techniques. 

 

See this for a list of sample buffer tolerances.

 

ESI or APCI:  depending on how clean the sample is (i.e. free of salts, etc.) and how long you want to analyze the sample, picomoles to micromoles of sample will be needed.  Preferred solvents include methanol, water and acetonitrile.

For direct infusion via the ESI or APCI probes bring at least 100 mL of sample.

For nanospray infusion bring 10 mL of sample.

 

EI:  generally about 10 mL of a 100 mM sample is adequate.  Acceptable solvents include methanol, chloroform, methylene chloride and hexane; avoid water.

Data:  Data from the LCQ and GCQ are easily pasted into other applications, e.g. Word, PowerPoint or Acrobat.  All data derived from staff analyses are backed up to CD for your convenience only.  Users are strongly encouraged to create their own backups.  This facility is not responsible for lost data!  Users are solely responsible for backing up data from their own analyses.